The Radioactive singers and fun. frontman will top the bill of the All is One Orlando Unity Concert, which takes place at Hard Rock Live Orlando in Florida on Wednesday (22Jun16).
"THIS WEDNESDAY w/@nateruessmusic in Orlando," Imagine Dragons wrote on Twitter. "100 per cent (of) proceeds to victims & families...help us spread the word..."
All of the funds from the show will benefit the OneOrlando Fund, which was set up following the shooting on 12 June (16), when Omar Mateen opened fire inside gay nightclub Pulse, killing 49 people and injuring more than 50.
The fund gives aid to the survivors, and victims and their families affected by the tragedy, which was named the deadliest mass shooting in U.S. history.
They're not the only stars raising funds - Christina Aguilera is donating her earnings from U.S. iTunes downloads of her song Change for the next three months to the National Compassion Fund.
Meanwhile, Broadway actors such as Idina Menzel, Lin-Manuel Miranda, and Audra MCDonald plus stars including Sarah Jessica Parker, Matthew Broderick, Carole King, Kristen Bell, and Gloria Estefan recorded a cover of Burt Bacharach and Hal David's 1965 hit What the World Needs Now Is Love. Sales of the track will benefit the LGBT Center of Central Florida.