The Titanium hitmaker performed at Pacha Ibiza as part of his F**k Me I'm Famous residency in May (15) and video footage from the appearance shows dancers wearing Native American headdresses dancing around horses.
Deadmau5 has now taken to Twitter.com to criticise Guetta over the performance, insisting DJ sets should not feature live animals.
In a series of messages on the social media site, he writes, "im sure the horse really appriciated (sic) it. stupid f**ks. f**k @davidguetta and f**k @pacha".
"for real... horses belong on a farm, not in a s**tty overpriced nightclub to be subjected to a s**tty overpaid DJ. @pacha".
Guetta has yet to respond to Deadmau5's comments.