The stars each alternated lines from a script, which included: ''We appreciate the dialogue. We see the effort from Hollywood but we need more. We are here. We've been here''.
'Mary Poppins Returns' actor Lin-Manuel asked: ''Do you see me in this industry?''
Billy said: ''We are more than a splash of colour on your white canvas.''
And Isis said: ''We're not your quota,.''
The group argued diverse stars should not be seen as a ''quota'' but instead considered ''quotable''.
They also asked: ''Would you rather breathe life into stereotypes or stand up against them? Because what I know of my culture isn't who you portray me to be.
''We're not your quota. We are quotable. So show me -- me. Not your me. Me.''
The PSA - which was created by the Alliance for Inclusive and Multicultural Marketing (AIMM) - concluded that it was ''time'' to unite to create change.
It said: ''Let's come together so we can create waves of change. It's time, see all.''
Model Isis shared a clip from the advert on her Twitter page and insisted there is still ''more work to be done'' in tackling the lack of diversity in showbiz.
She wrote: ''The Emmys should be applauded for the 33% increase in black nominees this year; but there's much more work to be done. It's time for the entertainment industry to have more diverse and fair cultural representation in programming, advertising and recognition.''
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