A grenade launcher wielded by Al Pacino in Scarface is expected to fetch up to $30,000 (£18,750), and John Belushi's famous Ray-Ban shades from The Blues Brothers are due to sell for up to $20,000 (£12,500).
A script for multi-Oscar winning mob classic The Godfather has a top estimate of $6,000 (£3,750), and other highly-collectible items going under the hammer include Michelle Pfeiffer's Catwoman bodysuit from Batman Returns, Ben Affleck's military uniform from Pearl Harbor, and an outfit sported by Sharon Stone in Martin Scorsese's Casino.
The auction, due to take place next month (Nov15), will features dozens of items being sold off by movie memorabilia experts at specialist props company The Golden Closet.
One of the most expensive lots is the 1924 Harold Lloyd Silver Ghost Rolls-Royce car used in Humphrey Bogart and Audrey Hepburn's 1955 film Sabrina and The Comic, which has a top estimate of $600,000 (£375,000).
Other star items in the sale are Leonardo DiCaprio's prison uniform from Catch Me If You Can, a suit worn by Orson Wells in his groundbreaking classic Citizen Kane, a gun wielded by Arnold Schwarzenegger in The Terminator, John Wayne's gun belt, and a pair of Frank Sinatra's boots.
There is also an array of outfits from hit TV shows including The Sopranos, Happy Days, The Dukes Of Hazzard, and Mad Men.
A spokesman for Julien's Auctions, which is staging the sale, says, "The Collection of The Golden Closet is an amazing story of Hollywood from the Golden Era to the present day. Some of the greatest film scenes and memorable television hits are represented in this massive collection. We are very excited for this incredible auction in November."
The sale is due to take place on 20 November (15).