Jimmy Fallon fears "deafening silence" from the 'Tonight Show' audience.

The 50-year-old comedian has hosted the TV talk-show since 2014, but Jimmy admits that being greeted by silence remains his "biggest" worry.

Asked to name his biggest fear on a new episode of 'Hot Ones', Jimmy replied: "As a comedian, silence.

"The deafening silence is my biggest fear, crying on camera which is happening now, my biggest fear. Probably my biggest fear is giving up in front of the American public and I’m not doing that."

Jimmy previously starred on 'Saturday Night Live' and he's admitted to having particularly fond memories of making a sketch with Justin Timberlake.

The comedian shared: "He’s one of the most talented human beings on earth, he’s a perfectionist.

"I remember we were doing 'SNL', and we’re doing the Barry Gibb talk show, so we’re about to go, like 20 seconds to air, and he goes, ‘Hey Jimmy remember, hit that harmony,’ and I go, ‘Yeah, I will,’ and he squeezed my hand and goes, ‘I’m serious dude, remember to hit that harmony.’ And I’m like calm down dude, we’re dressed like the Bee Gees, for that second I felt what it was like to be Lance Bass."

Meanwhile, Jimmy previously insisted that he's never been motivated by money.

Asked what advice he would give to someone who wants to follow in his footsteps, the comedian told USA Today: "Don’t do it for money. You’ll never make money. Do it because you like it and you like what you’re doing.

"And then, the secret is, you may end up making money. But don’t go into it thinking that you’re doing this for money, because I never thought about money. Never, ever.

"Don’t worry about money, just do it because it’s fun and it’s interesting for you. And keep it interesting and stay creative and keep dreaming and keep believing and it will all fall into place."